The investigation of complex communication in cellular networks requires superior measurement tools than those available to date. Electrode arrays integrated onto silicon electronics are increasingly used to measure the electrical activity of cells in an automated and highly parallelized fashion, but they are restricted to recording extracellular potentials. Here, we report on an array of TiN electrodes built using standard silicon electronics for intracellular action potential recording. Intracellular access, possible at each of the 16384 electrodes on the chip, was accomplished by local membrane electroporation using electrical stimulation with subcellular, micrometer-sized electrodes. Access to the cell interior was transient and could be tuned in duration by adapting the electroporation protocol. Intracellular sensing was found to be minimally invasive in the short and long-term, allowing consecutive intracellular recordings from the same cell over the course of days. Finally, we applied this method to investigate the effect of an ion channel blocker on cardiac electrical activity. This technique opens the door to massively parallel, long-term intracellular recording for fundamental electrophysiology and drug screening. {\textcopyright} 2012 The Royal Society of Chemistry.
Braeken, D, Jans, D, Huys, R, Stassen, A, Collaert, N, Hoffman, L, Eberle, W, Peumans, P & Callewaert, G 2012, 'Open-cell recording of action potentials using active electrode arrays', Lab on a Chip, vol. 12, no. 21, pp. 4397-4402. https://doi.org/10.1039/c2lc40656j
Braeken, D., Jans, D., Huys, R., Stassen, A., Collaert, N., Hoffman, L., Eberle, W., Peumans, P., & Callewaert, G. (2012). Open-cell recording of action potentials using active electrode arrays. Lab on a Chip, 12(21), 4397-4402. https://doi.org/10.1039/c2lc40656j
@article{59ce07a72a4e4328bf2337add4b37fa2,
title = "Open-cell recording of action potentials using active electrode arrays",
abstract = "The investigation of complex communication in cellular networks requires superior measurement tools than those available to date. Electrode arrays integrated onto silicon electronics are increasingly used to measure the electrical activity of cells in an automated and highly parallelized fashion, but they are restricted to recording extracellular potentials. Here, we report on an array of TiN electrodes built using standard silicon electronics for intracellular action potential recording. Intracellular access, possible at each of the 16384 electrodes on the chip, was accomplished by local membrane electroporation using electrical stimulation with subcellular, micrometer-sized electrodes. Access to the cell interior was transient and could be tuned in duration by adapting the electroporation protocol. Intracellular sensing was found to be minimally invasive in the short and long-term, allowing consecutive intracellular recordings from the same cell over the course of days. Finally, we applied this method to investigate the effect of an ion channel blocker on cardiac electrical activity. This technique opens the door to massively parallel, long-term intracellular recording for fundamental electrophysiology and drug screening. {\textcopyright} 2012 The Royal Society of Chemistry.",
keywords = "silicon, titanium derivative, titanium nitride, unclassified drug, action potential, animal cell, animal tissue, article, cell expansion, cell stimulation, drug screening, electrode, electrophysiology, electroporation, electrostimulation, embryo, female, filtration, intracellular recording, molecular size, mouse, nonhuman, priority journal",
author = "Dries Braeken and Danny Jans and Roeland Huys and Andim Stassen and Nadine Collaert and Luis Hoffman and Wolfgang Eberle and Peter Peumans and Geert Callewaert",
year = "2012",
month = nov,
day = "7",
doi = "10.1039/c2lc40656j",
language = "English",
volume = "12",
pages = "4397--4402",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "21",
}