Very-large scale integration and micro-machining have enabled the development of novel platforms for advanced and automated examination of cells and tissues in vitro. In this paper, we present a CMOS chip designed in a commercial 0.18 μm technology with integrated micro-syringes combined with micro-nail shaped electrodes and readout electronics. The micro-syringes could be individually addressed by a through-wafer micro-fluidic channel with an inner diameter of 1 μm. We demonstrated the functionality of the micro-fluidic access by diffusion of fluorescent species through the channels. Further, hippocampal neurons were cultured on top of an array of micro-syringes, and focused ion beam-scanning electron microscopy cross-sections revealed protrusion of the cells inside the channels, creating a strong interface between the membrane and the chip surface. This principle demonstrates a first step towards a novel type of automated in vitro platforms, allowing local delivery of substances to cells or advanced planar patch clamping.
Huys, R, Braeken, D, Micholt, L, Jans, D, Stassen, A, Collaert, N, Loo, J, Bartic, C, Verstreken, K & Eberle, W 2011, 'Micro-sized syringes for single-cell fluidic access integrated on a micro-electrode array CMOS chip.', Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, vol. 2011, pp. 7650-7653.
Huys, R., Braeken, D., Micholt, L., Jans, D., Stassen, A., Collaert, N., Loo, J., Bartic, C., Verstreken, K., & Eberle, W. (2011). Micro-sized syringes for single-cell fluidic access integrated on a micro-electrode array CMOS chip. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, 2011, 7650-7653.
@article{58c073ddc2ea4c2e9ecaf856a710e47a,
title = "Micro-sized syringes for single-cell fluidic access integrated on a micro-electrode array CMOS chip.",
abstract = "Very-large scale integration and micro-machining have enabled the development of novel platforms for advanced and automated examination of cells and tissues in vitro. In this paper, we present a CMOS chip designed in a commercial 0.18 μm technology with integrated micro-syringes combined with micro-nail shaped electrodes and readout electronics. The micro-syringes could be individually addressed by a through-wafer micro-fluidic channel with an inner diameter of 1 μm. We demonstrated the functionality of the micro-fluidic access by diffusion of fluorescent species through the channels. Further, hippocampal neurons were cultured on top of an array of micro-syringes, and focused ion beam-scanning electron microscopy cross-sections revealed protrusion of the cells inside the channels, creating a strong interface between the membrane and the chip surface. This principle demonstrates a first step towards a novel type of automated in vitro platforms, allowing local delivery of substances to cells or advanced planar patch clamping.",
keywords = "oxide, animal, article, cell culture, chemistry, cytology, electronics, fluorescence, devices, microelectrode, microfluidic analysis, mouse, nerve cell, scanning electron microscopy, semiconductor, single cell analysis, syringe",
author = "Roeland Huys and Dries Braeken and Liesbeth Micholt and Danny Jans and Andim Stassen and Nadine Collaert and Josine Loo and Carmen Bartic and Kris Verstreken and Wolfgang Eberle",
year = "2011",
month = jan,
day = "1",
language = "English",
volume = "2011",
pages = "7650--7653",
journal = "Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference",
issn = "1557-170X",
}