Time-resolved fluorescence spectroscopy is a promising and versatile spectroscopy technique. While conventional fluorescence spectroscopy is concerned with the spectral features of fluorescence emission, time-resolved fluorescence spectroscopy (TRFS) also captures the temporal behavior of the excited molecules. In addition to applications in rapid medical diagnoses, several applications were reported in forensics, chemical analysis and biology. The existing TRFS systems are based on complex optical setups which results in bulky and large systems. Therefore, using them in practice is still not feasible. In this work, we present a miniaturized system that combines a compact Czerny-Turner based spectrometer layout with our high-speed time-gated image sensor of 32×32 pixels based on the Current-Assisted Photonic Sampler (CAPS) sensor. The system operates in the visible wavelength range with a high spectral resolution. The novel image sensor is capable of measuring down to sub-nanosecond lifetimes. The proof-of-concept module was assembled and characterized for three fluorophores.
Gasser, A, Shcheglov, A, Lapauw, T, Van Den Dries, T, Ingelberts, H, Hoving, W, Aalders, M & Kuijk, M 2022, Miniaturized Time-Resolved Fluorescence Spectrometer System. in LE Busse, Y Soskind & PC Mock (eds), Photonic Instrumentation Engineering IX. vol. 12008, 120080B, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12008, SPIE, California, USA, SPIE Photonics West 2022, San Francisco, California, United States, 22/01/22. https://doi.org/10.1117/12.2609535
Gasser, A., Shcheglov, A., Lapauw, T., Van Den Dries, T., Ingelberts, H., Hoving, W., Aalders, M., & Kuijk, M. (2022). Miniaturized Time-Resolved Fluorescence Spectrometer System. In L. E. Busse, Y. Soskind, & P. C. Mock (Eds.), Photonic Instrumentation Engineering IX (Vol. 12008). Article 120080B (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12008). SPIE. https://doi.org/10.1117/12.2609535
@inproceedings{ca91b65df84845cdb0dc1ba4304a5aa2,
title = "Miniaturized Time-Resolved Fluorescence Spectrometer System",
abstract = "Time-resolved fluorescence spectroscopy is a promising and versatile spectroscopy technique. While conventional fluorescence spectroscopy is concerned with the spectral features of fluorescence emission, time-resolved fluorescence spectroscopy (TRFS) also captures the temporal behavior of the excited molecules. In addition to applications in rapid medical diagnoses, several applications were reported in forensics, chemical analysis and biology. The existing TRFS systems are based on complex optical setups which results in bulky and large systems. Therefore, using them in practice is still not feasible. In this work, we present a miniaturized system that combines a compact Czerny-Turner based spectrometer layout with our high-speed time-gated image sensor of 32×32 pixels based on the Current-Assisted Photonic Sampler (CAPS) sensor. The system operates in the visible wavelength range with a high spectral resolution. The novel image sensor is capable of measuring down to sub-nanosecond lifetimes. The proof-of-concept module was assembled and characterized for three fluorophores.",
keywords = "Fluorescence lifetime, TRFS,, Time-Gated Imaging Sensors, CAPS Sensor Systems, Czerny-Turner Spectrometer, Miniaturization.",
author = "Anas Gasser and Artem Shcheglov and Thomas Lapauw and {Van Den Dries}, Thomas and Hans Ingelberts and Willem Hoving and Maurice Aalders and Maarten Kuijk",
year = "2022",
month = mar,
day = "5",
doi = "10.1117/12.2609535",
language = "English",
isbn = "978-1-5106-4887-6",
volume = "12008",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Busse, {Lynda E.} and Yakov Soskind and Mock, {Patrick C.}",
booktitle = "Photonic Instrumentation Engineering IX",
address = "United States",
note = "SPIE Photonics West 2022 ; Conference date: 22-01-2022 Through 27-01-2022",
url = "https://spie.org/conferences-and-exhibitions/photonics-west",
}