Fluorescence molecular imaging (FMI) is becoming a powerful tool to improve surgical precision and diagnostics. Although significant advances have been made in imaging technology and contrast agents, debate continues regarding suitable standards for FMI. Standardizing all FMI aspects is crucial for adoption in clinical procedures and routine practice. Currently, the lack of clear-cut guidelines on FMI study reporting complicates comparison between studies and impedes standardization. This work presents community-driven REFLECT guidelines for FMI study reporting for fluorescence-guided surgery and interventions, covering preclinical, translational, and clinical studies. It aims to improve quality of reporting, enable comparison between studies, and enhance interpretation of results. The resulting structured checklist encompasses all essential details for reporting on the contrast agent, imaging device, imaging protocol, and image processing and analysis methods. Adoption of this framework is encouraged to enhance reproducibility and establish alignment with standardization efforts in the field - thus fostering advancements in FMI.
Keizers, B, Stroet, M, Ali, M, Floru, S, Saliën, J, Mezzanotte, L, Delikatny, E, Gibbs, S, Giuliani, S, Gioux, S, Ingelberts, H, Kruijff, S, Ntziachristos, V, LaRochelle, E, Rogalla, S, Rosenthal, E, Samkoe, K, Tichauer, K, Vahrmeijer, AL, Witjes, M, Voskuil, F, Gorpas, D, Hernot, S & van der Zaag, P 2025, 'Reflect: reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies', npj Imaging. https://doi.org/10.1038/s44303-025-00125-8
Keizers, B., Stroet, M., Ali, M., Floru, S., Saliën, J., Mezzanotte, L., Delikatny, E., Gibbs, S., Giuliani, S., Gioux, S., Ingelberts, H., Kruijff, S., Ntziachristos, V., LaRochelle, E., Rogalla, S., Rosenthal, E., Samkoe, K., Tichauer, K., Vahrmeijer, A. L., ... van der Zaag, P. (2025). Reflect: reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies. npj Imaging, Article 62 . https://doi.org/10.1038/s44303-025-00125-8
@article{d939e0e7be3c4f15bb4a83b19aa85aba,
title = "Reflect: reporting guidelines for preclinical, translational and clinical fluorescence molecular imaging studies",
abstract = "Fluorescence molecular imaging (FMI) is becoming a powerful tool to improve surgical precision and diagnostics. Although significant advances have been made in imaging technology and contrast agents, debate continues regarding suitable standards for FMI. Standardizing all FMI aspects is crucial for adoption in clinical procedures and routine practice. Currently, the lack of clear-cut guidelines on FMI study reporting complicates comparison between studies and impedes standardization. This work presents community-driven REFLECT guidelines for FMI study reporting for fluorescence-guided surgery and interventions, covering preclinical, translational, and clinical studies. It aims to improve quality of reporting, enable comparison between studies, and enhance interpretation of results. The resulting structured checklist encompasses all essential details for reporting on the contrast agent, imaging device, imaging protocol, and image processing and analysis methods. Adoption of this framework is encouraged to enhance reproducibility and establish alignment with standardization efforts in the field - thus fostering advancements in FMI.",
author = "Bas Keizers and Marcus Stroet and Meedie Ali and Sam Floru and Jelena Sali{\"e}n and Laura Mezzanotte and Edward Delikatny and Summer Gibbs and Stefano Giuliani and Sylvain Gioux and Hans Ingelberts and Schelto Kruijff and Vasilis Ntziachristos and Ethan LaRochelle and Stephan Rogalla and Eben Rosenthal and Kimberley Samkoe and Kenneth Tichauer and Vahrmeijer, {Alexander Lucas} and Max Witjes and Floris Voskuil and Dimitris Gorpas and Sophie Hernot and {van der Zaag}, Pieter",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",
year = "2025",
month = nov,
day = "25",
doi = "10.1038/s44303-025-00125-8",
language = "English",
journal = "npj Imaging",
issn = "2948-197X",
publisher = "Nature Research",
}