An in-house developed Label-free Immobilization-free mm-wave sensor has shown its potential in various applications[1][2]. We present the experimental results in the field of HPLC(high performance liquid chromatography), protein binding and protein crystallization. In HPLC measurement, the results of mm-wave sensor is compared with UV-sensor integrated on the HPLC machine. It is shown that for both UV and non-UV absorbing samples, mm-wave sensor can be used as detection tool without labelling. By probing the protein molecules and the hydration shell around them, mm-wave sensor is also sensitive to the existence of protein in a solution with a current detection limit of 1 mg/ml. In protein binding experiment, the mm wave sensor detects the difference between binding and non-binding groups. Last but not least, the sensor demonstrates its capability to track protein crystallization process and the potential to derive induction time.
Zhang, Y, Declerck, S, Stroobants, S, Pandey, G, He, G, Mangelings, D, Matvejev, V, Stiens, J, Maes, D, Muyldermans, S, Mihăilescu, D & Mernea, MA 2016, 'Experimental validation of a Label-free Immobilization-free mm wave sensor in different biological and pharmaceutical applications', the International Conference on Semiconductor Mid-IR Materials and Optics SMMO2016, lisbon, Portugal, 21/03/16 - 24/03/16.
Zhang, Y., Declerck, S., Stroobants, S., Pandey, G., He, G., Mangelings, D., Matvejev, V., Stiens, J., Maes, D., Muyldermans, S., Mihăilescu, D., & Mernea, M. A. (2016). Experimental validation of a Label-free Immobilization-free mm wave sensor in different biological and pharmaceutical applications. the International Conference on Semiconductor Mid-IR Materials and Optics SMMO2016, lisbon, Portugal.
@conference{409b13a7c9324e4c88a36eb1bc3ed941,
title = "Experimental validation of a Label-free Immobilization-free mm wave sensor in different biological and pharmaceutical applications",
abstract = "An in-house developed Label-free Immobilization-free mm-wave sensor has shown its potential in various applications[1][2]. We present the experimental results in the field of HPLC(high performance liquid chromatography), protein binding and protein crystallization. In HPLC measurement, the results of mm-wave sensor is compared with UV-sensor integrated on the HPLC machine. It is shown that for both UV and non-UV absorbing samples, mm-wave sensor can be used as detection tool without labelling. By probing the protein molecules and the hydration shell around them, mm-wave sensor is also sensitive to the existence of protein in a solution with a current detection limit of 1 mg/ml. In protein binding experiment, the mm wave sensor detects the difference between binding and non-binding groups. Last but not least, the sensor demonstrates its capability to track protein crystallization process and the potential to derive induction time.",
author = "Yuchen Zhang and Sven Declerck and Sander Stroobants and Gokarna Pandey and Guoqiang He and Debby Mangelings and Vladimir Matvejev and Johan Stiens and Dominique Maes and Serge Muyldermans and Dan Mih{\u a}ilescu and Mernea, {Maria Alexandra}",
year = "2016",
month = mar,
day = "22",
language = "English",
note = "the International Conference on Semiconductor Mid-IR Materials and Optics SMMO2016 ; Conference date: 21-03-2016 Through 24-03-2016",
}