Publication Details
Overview
 
 
Yuchen Zhang, Yuchen Zhang, Sven Declerck, Sven Declerck, Sander Stroobants, Sander Stroobants, Gokarna Pandey, Gokarna Pandey, Guoqiang He, Guoqiang He, Debby Mangelings, Debby Mangelings, Vladimir Matvejev, Vladimir Matvejev, Johan Stiens, Johan Stiens, Dominique Maes, Dominique Maes, Serge Muyldermans, Serge Muyldermans, Dan Mihăilescu, Dan Mihăilescu, Maria Alexandra Mernea, Maria Alexandra Mernea
 

Unpublished contribution to conference

Abstract ■

An in-house developed Label-free Immobilization-free mm-wave sensor has shown its potential in various applications[1][2]. We present the experimental results in the field of HPLC(high performance liquid chromatography), protein binding and protein crystallization. In HPLC measurement, the results of mm-wave sensor is compared with UV-sensor integrated on the HPLC machine. It is shown that for both UV and non-UV absorbing samples, mm-wave sensor can be used as detection tool without labelling. By probing the protein molecules and the hydration shell around them, mm-wave sensor is also sensitive to the existence of protein in a solution with a current detection limit of 1 mg/ml. In protein binding experiment, the mm wave sensor detects the difference between binding and non-binding groups. Last but not least, the sensor demonstrates its capability to track protein crystallization process and the potential to derive induction time.

Reference ■