Publication Details
Overview
 
 
Yuchen Zhang, Sven Declerck, Sander Stroobants, Gokarna Pandey, Guoqiang He, Debby Mangelings, Vladimir Matvejev, Johan Stiens, Dominique Maes, Serge Muyldermans, Dan Mihăilescu, Maria Alexandra Mernea
 

Unpublished contribution to conference

Abstract 

An in-house developed Label-free Immobilization-free mm-wave sensor has shown its potential in various applications[1][2]. We present the experimental results in the field of HPLC(high performance liquid chromatography), protein binding and protein crystallization. In HPLC measurement, the results of mm-wave sensor is compared with UV-sensor integrated on the HPLC machine. It is shown that for both UV and non-UV absorbing samples, mm-wave sensor can be used as detection tool without labelling. By probing the protein molecules and the hydration shell around them, mm-wave sensor is also sensitive to the existence of protein in a solution with a current detection limit of 1 mg/ml. In protein binding experiment, the mm wave sensor detects the difference between binding and non-binding groups. Last but not least, the sensor demonstrates its capability to track protein crystallization process and the potential to derive induction time.

Reference