Mernea, M. A., Vasile, I., Calborean, O., Stiens, J., & Mihăilescu, D. (2015). Molecular modelling on GHz-THz sensing of camel single domain antibodies binding to lysozyme. In National Conference of the Romanian Society of Biophysics (2015 ed.).

@inbook{cd24b2a32ec548d481dc3c8deed32a63,
title = "Molecular modelling on GHz-THz sensing of camel single domain antibodies binding to lysozyme",
abstract = "Camel single domain antibodies are antibody fragments with a low molecular weight (~15 kDa) that are fully capable to bind their targets. Due to their high stability, these small antibodies are of great interest for biosensing applications, biotechnological use and more importantly for therapeutic use, in fighting oncological, neurodegenerative, infectious and inflammatory diseases. Here we addressed the issue of sensing the binding of camel single domain antibodies to a target protein, namely hen egg white lysozyme. Spectroscopic experiments performed in the Gigahetz (GHz) – Terahertz (THz) frequency domains showed a significant signal change upon the formation of antibody – lysozyme complexes. Molecular modelling was used in order to understand the experimental results at the molecular level. The spectra of the proteins and their hydration shells were simulated based on the molecular dynamics trajectories [1] of dilute model solutions comprising: i) the antibody – lysozyme complex [2], ii) lysozyme and iii) the antibody molecule. Theoretical results on the solutions components were used to estimate the absorptions of solutions with the lysozyme – antibody ratios used in experiments. ",
keywords = "Biophysics",
author = "Mernea, {Maria Alexandra} and Ionut Vasile and Octavian Calborean and Johan Stiens and Dan Mih{\u a}ilescu",
year = "2015",
month = jun,
day = "4",
language = "English",
booktitle = "National Conference of the Romanian Society of Biophysics",
edition = "2015",
}