Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
Willaert, R, Vanden Boer, PK, Malovichko, A, Alioscha-Perez, M, Radotic, K, Bartolic, D, Kalauzi, A, Villalba, MI, Sanglard, D, Dietler, G, Sahli, H & Kasas, S 2020, 'Single yeast cell nanomotions correlate with cellular activity', Science Advances, vol. 6, no. 26, eaba3139, pp. 1-8. https://doi.org/10.1126/sciadv.aba3139
Willaert, R., Vanden Boer, P. K., Malovichko, A., Alioscha-Perez, M., Radotic, K., Bartolic, D., Kalauzi, A., Villalba, M. I., Sanglard, D., Dietler, G., Sahli, H., & Kasas, S. (2020). Single yeast cell nanomotions correlate with cellular activity. Science Advances, 6(26), 1-8. Article eaba3139. https://doi.org/10.1126/sciadv.aba3139
@article{b6fb8367a424421891c2b1e968ebc5d1,
title = "Single yeast cell nanomotions correlate with cellular activity",
abstract = "Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.",
keywords = "yeast, Single Cells, nanomotion, nanotechnology, cell biology, Antifungals, Antifungal susceptibility testing",
author = "Ronnie Willaert and {Vanden Boer}, {Pieterjan Kris} and Anton Malovichko and Mitchel Alioscha-Perez and Ksenija Radotic and Dragana Bartolic and Aleksandar Kalauzi and Villalba, {Maria Ines} and Dominique Sanglard and Giovanni Dietler and Hichem Sahli and Sandor Kasas",
note = "Funding Information: This work was supported by the Belgian Federal Science Policy Office (Belspo) and the European Space Agency (ESA) PRODEX program (Yeast Bioreactor project). The Research Council of the Vrije Universiteit Brussel (Belgium) support the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO) and the Strategic Research Program (project SRP11-M3D2). Swiss members of the team were funded by the Swiss National Grants 200021-144321, 407240-167137, and CRSII5_173863; the Gebert R{\"u}f Stiftung GRS-024/14; and NASA NNH16ZDA001N-CLDTCH. Serbian members of the team were funded by the grant 173017 from the Ministry of Education, Science and Technological Development of the Republic of Serbia. Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jun,
day = "24",
doi = "10.1126/sciadv.aba3139",
language = "English",
volume = "6",
pages = "1--8",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "26",
}