Coronary artery disease (CAD) contributes to millions of deaths each year. The identification of vulnerable plaques is essential to the diagnosis of CAD but is challenging. Molecular probes can improve the detection of these plaques using intravascular imaging methods. Fluorescence lifetime sensing is a safe and robust method to image these molecular probes. We present two variations of an optical system for intravascular near-infrared (NIR) fluorescence lifetime sensing through a multimode fiber. Both systems are built around a recently developed fast and efficient CMOS detector, the current-assisted photonic sampler (CAPS) that is optimized for sub-nanosecond NIR fluorescence lifetime sensing. One system mimics the optical setup of an epifluorescence microscope while the other uses a practical fiber optic coupler to separate fluorescence excitation and emission. We test both systems by measuring the lifetime of several NIR dyes in DMSO solutions and we show that these systems are capable of detecting lifetimes of solutions with concentrations down to 370 nM and this with short acquisition times. These results are compared with time-correlated single photon counting (TCSPC) measurements for reference. {\textcopyright} (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ingelberts, H, Kuijk, M, Hernot, S, Debie, P & Lahoutte, T 2016, NIR fluorescence lifetime sensing through a multimode fiber for intravascular molecular probing. in Proc. SPIE 9887: Biophotonics: Photonic Solutions for Better Health Care V. vol. 9887, 58, SPIE, pp. 1-7, SPIE Photonics Europe 2016, Brussels, Belgium, 3/04/16.
Ingelberts, H., Kuijk, M., Hernot, S., Debie, P., & Lahoutte, T. (2016). NIR fluorescence lifetime sensing through a multimode fiber for intravascular molecular probing. In Proc. SPIE 9887: Biophotonics: Photonic Solutions for Better Health Care V (Vol. 9887, pp. 1-7). Article 58 SPIE.
@inproceedings{32360c5806364421b1bec2b370171547,
title = "NIR fluorescence lifetime sensing through a multimode fiber for intravascular molecular probing",
abstract = "Coronary artery disease (CAD) contributes to millions of deaths each year. The identification of vulnerable plaques is essential to the diagnosis of CAD but is challenging. Molecular probes can improve the detection of these plaques using intravascular imaging methods. Fluorescence lifetime sensing is a safe and robust method to image these molecular probes. We present two variations of an optical system for intravascular near-infrared (NIR) fluorescence lifetime sensing through a multimode fiber. Both systems are built around a recently developed fast and efficient CMOS detector, the current-assisted photonic sampler (CAPS) that is optimized for sub-nanosecond NIR fluorescence lifetime sensing. One system mimics the optical setup of an epifluorescence microscope while the other uses a practical fiber optic coupler to separate fluorescence excitation and emission. We test both systems by measuring the lifetime of several NIR dyes in DMSO solutions and we show that these systems are capable of detecting lifetimes of solutions with concentrations down to 370 nM and this with short acquisition times. These results are compared with time-correlated single photon counting (TCSPC) measurements for reference. {\textcopyright} (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.",
author = "Hans Ingelberts and Maarten Kuijk and Sophie Hernot and Pieterjan Debie and Tony Lahoutte",
year = "2016",
month = apr,
day = "27",
language = "English",
isbn = "9781510601321 ",
volume = "9887",
pages = "1--7",
booktitle = "Proc. SPIE 9887",
publisher = "SPIE",
address = "United States",
note = "SPIE Photonics Europe 2016 : Optics, Photonics and Digital Technologies for Imaging Applications IV ; Conference date: 03-04-2016 Through 07-04-2016",
url = "http://spie.org/conferences-and-exhibitions/photonics-europe",
}