Bottenberg, P., Henin, L., Boca, A.-C., Postnov, A., Wasek, A., De Clerck, N., & Truyen, B. (2003). Application of desktop micro-CT imaging as gold standard in caries diagnosis. Journal of Dental Research, 82((Spec Iss B)), B386-B386. https://doi.org/10.1177/00220345030820SA01

@article{770d1e726e64491f9b7276757c7e9619,
title = "Application of desktop micro-CT imaging as gold standard in caries diagnosis",
abstract = "Objectives: Conventionally, 'gold standards' used in the evaluation of different caries diagnosis techniques are based on histological sectioning. Such a procedure, however, requires cumbersome sample preparation, and induces tissue loss and irreversible sample destruction. Micro Computed Tomograhy (micro-CT) allows nondestructive imaging of the internal tooth structure, with information being visualized either as discrete sections or as 3D volume renderings.Methods: Micro-CT images were compared to clinical and histological evaluations of 16 extracted teeth, encompassing different pathologies. Clinical evaluation was performed by a panel of three dentists and three graduate students on models containing 3 teeth, among which the central one was previously evaluated by micro-CT. Caries was rated using 6 graduations (sound, probably sound, white/brown spot, enamel caries, dentine caries limited to half of dentin depth, deep dentine caries). Micro-CT scans were acquired using a desktop system (SkyScan-1072, Belgium), based on a combination of X-ray projection microscopy and tomographic data reconstruction. In this system, an air-cooled X-ray point source (20-130 kV / 300 µA, focal spot size ~8 mm) illuminates the object with a divergent beam, and magnified shadow pictures are detected by a 2D X-ray CCD camera (512 x 512 pixels). Cross-sectional images were reconstructed at a spatial resolution of 14µm3, using a Feldkamp cone-beam algorithm. Histological evaluation was performed using a Leitz hard tissue microtome (600 µm sections) and a stereomicroscope. Spearman{\textquoteright}s rank correlation analysis was performed between the histological, micro-CT, and clinical outcomes.Results: The micro-CT analysis demonstrated good agreement with histological measurements (r = 0.89, p < 0.001), whilst the clinical evaluations were found to be much less correlated with the micro-CT analysis (r = 0.39, p < 0.001) and histological data (r = 0.42, p < 0.001).Conclusion: Micro-CT imaging was shown to provide an interesting alternative to histological sectioning in the evaluation of caries diagnosis. As a nondestructive technique, micro-CT allows longitudinal evaluation of the same tooth material.",
keywords = "cariology, gold standard, histological sectioning, micro computed tomography",
author = "Peter Bottenberg and Lucia Henin and Ana-Cristina Boca and Andrei Postnov and Adam Wasek and {De Clerck}, Nora and Bart Truyen",
year = "2003",
month = mar,
day = "1",
doi = "10.1177/00220345030820SA01",
language = "English",
volume = "82",
pages = "B386--B386",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "(Spec Iss B)",
note = "81st General Session of the International Association for Dental Research ; Conference date: 25-06-2003 Through 29-06-2003",
}