Publication Details
Bart Truyen, Aleksandra Lamentowicz, Andrei Postnov, Cristina Boca, Peter Bottenberg, Lucia Henin, Michael Penkowski, Nora De Clerck, Barbara Kochanska, Jan Cornelis

Caries Research

Contribution To Journal


Evidence was found that local application of stannous fluoride (SnF2) may lead to an increased radiographic density in approximal lesions [Halse A et al.: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1990 69: 378–381]. However, few conclusive results were provided as to the actual penetration depth, the uptake in lesions without clinical signs of cavitation, or its retention properties. To deliver an answer to these questions, and to compare the penetration properties of SnF2 with those of YbCl3, 6 extracted premolars, carefully selected to encompass 12 proximal surfaces with histologically confirmed dentinal and enamel lesions, were subjected to x-ray microtomography (XMT). After having their apices sealed, all teeth were scanned with a SkyScan-1076 desktop XMT system (Skyscan, Aartselaar, Belgium) at a resolution of 18µm3, to determine the actual extent of demineralization. Two groups of 3 teeth were immersed for 1 minute in saturated SnF2 (300g/L, equiv. to 227g/L Sn(II)) and YbCl3 (2170g/L, equiv. to 969g/L Yb(III)) solutions, respectively, and a new set of XMT scans was acquired. Subsequently, each tooth was rinsed in water for 30 seconds and subjected to another XMT scanning, which was repeated after 2 days of storage in water. In this way, the non-destructive nature of XMT combined with its tomographic visualization, provided a unique opportunity to investigate possibly adverse effects of rinsing on the contrasting. During scanning, the moisted teeth were temporarily removed from their individual storage containers, and air exposed. Subtraction was performed between pre- and post-contrasting recordings. Finally, conventional histological slices were prepared, and correlated with the cross-sectional XMT sections. For all 12 lesions, subtraction images revealed signs of radiographic contrasting. Previous speculations about the uptake of SnF2 in noncavitated enamel lesions were confirmed, despite the better penetration properties observed for YbCl3. Rinsing was found only to marginally affect the contrast retention. Contrast diminishment after 2 days indicates that YbCl3 contrasting is a reproducible procedure.